Search results for "Mercuric Chloride"

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Translocation of the nuclear autoantigen La to cell surface: assembly and disassembly with the extracellular matrix.

1991

La (SS-B) protein is known as one major antigenic target for autoantibodies from patients with certain autoimmune diseases such as Sjogren's syndrome or Lupus Erythematosus. La protein belongs to the so called "extractable nuclear antigens". Here we report that La antigen is not restricted to the nucleus as one might deduce from the exclusive nuclear staining pattern of patient anti-La antibodies but after stimulation of serum-starved cells with 10% fetal calf serum (FCS) appears and stays for at least 45 min at the outer surface of CV-1 cells being available for binding of anti-La antibodies. In addition we found that a minor part of La antigen associates with the extracellular fibronectin…

ImmunologyBiological Transport ActiveAutoimmunityBiologyIn Vitro TechniquesAutoantigensEpitopeExtracellular matrixEpitopesAntigenExtracellularImmunology and AllergyHumansNuclear proteinCells CulturedCell NucleusInflammationCell MembraneMolecular biologyExtracellular MatrixFibronectinBiochemistryMicroscopy FluorescenceRibonucleoproteinsCell cultureMercuric Chloridebiology.proteinElectrophoresis Polyacrylamide GelAntibodyAutoimmunity
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